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KMID : 0382619840040010277
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Hanyang Journal of Medicine
1984 Volume.4 No. 1 p.277 ~ p.289
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A study for Polymicrobial Infection in Mouse and Mixed Culture in Test Tube
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崔泰悅/Choi,m Tae Yeal
金春元/金箕洪/Kim, Choon Won/Kim, Ki Hong
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Abstract
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Polymicrobial infection is defined as infected state due to at least two different microbial organism including bacteria, virus, richettia, fungus, and parasite, and may be called mixed infection, superimposed infection, and secondary infection. The fusospirochetal disease which is simultaneous infection of Borrelia vincenti and Bacillus fusiformis is one of well known example of famous polymicrobial infection. Recently, clincian have interested polymicrobial infection due to 1) advance the technic of bacterial isolation and identification, especially anaerobic and nonfermented bacterias, 2) increased secondary infection due to wide use of antibacterial drug, 3) frequently convert to severe and incurable infectious disease. Microbial synergism may involve following interaction. Microorganisms can lower the resistance of the host and in so doing increasing the likehood of invasion by another microorganism. They may also facilitate colinization of potential hosts, provide elements that are essential to the gro-wth or increased the virulence of other microorganism. Many microorganisms diminish ability to resist invasion by secondary pathogens. They do so by altering celluar immunity, humoral immunity or the anatomy of the host. Author have studied basically for polymicrobial infection in mouse and mixed culture in test tube. Hospital pathogenic organism Staph. aureus (ATCC 25922), enteric nirmal flora E. coli(ATCC 25923),and enviromental flora S.marcescens (quality control, 1981) have been used. Inicial colony count of brain heart infusion broth in test tube is 1 x 10³/ml, and incubated at 37℃. A bacteremia have been induced in moused by intra-peritonial injection of individual and mixed bacteria.
The results are as follows:
1) Measurement of lethal dosage-50(LD-50), minimal lethal dosage (MLD), and injection dosage (Inj. Dos.) of individual bacteria in ICR-mouse.
1. E. Coli : LD-50 3.2x10??/ml, MLD 4x10??/m, and Inj. Dos. 4x10??/ml.
2. Staph. aureus: LD-50 3.2x10??/ml, MLD 1x10??/ml, and Inj. Dos. 1x10??/ml.
3. S. marcescens: LD-50 3x10?/ml, and Inj. Dos. 4x10??/ml.
(The vilurence of S. marcescens is strongest and follow to E. Coli, Staph. aureus.)
2) The colony count of individual and mixed bacterial culture in test tube.
1. E. coli: Growth pattern continuously increased to 6.4x10??/ml at 24hr.
2. Staph. aureus: Growth pattern shows standard growth cutve, and increased to 4.5x10??/ml at 24hr.
3. S. marcescens: Growth pattern shows continously increased to 1.3x10??/ml at 24hr.
(The growth rate of E. coli is the fastest, and follow to staph. aureus, S.marcescens)
4. E. coli and staph. aureus: Both growth pattern show standard growth curves, and increased to 1.4x10??/ml, at 24hr, in respectively.
(The growth of E. coli is superior to staph. aureus)
5. E. coli and S. marcescens: Both growth patterns show standard growth curves and increased to 1.8x10??/ml, 1.0x10??ml at 24hr, in respectively.
(The growth of E. coli superior to S. marcescens.)
6. Staph. suteus and S. mascescens: Both growth pattrns show continuously increased to 9.3x10??/ml at 24hr, in respectively.
7. E. coli, Staph. aureus, and S. marcescens: Three growh patterns show continuously increased to 1.1x10??/ml, 1.0x10??ml. 5.0x10??/ml at 24hr, in respectively.
(The fastest growth organism is E. coli and followed to staph. auteus and S.marcescens.)
3) The colony count in blood of polymicrobial infection of mouse.
1. E. coli: E. coli rapidly increase to 1.1x10??/ml at 24hr and rapidly decrdased to 0/ml at 8hr, but small amount bacteria have been survived at 24hr (the deathrate:0%)
2. Staph. aureus: Staph aureus rapidly increased to 4x10??.ml at 2hr and rapidly decreased to 1x10/ml at 8hr, and slowly decreased to 0/ml at 24hr(the death rate:0%)
3. S. marcesens: S. marcescens rapidly increased to 3x10??/ml at 24hr and rapidly decreased to 1.0x10/ml at 8hr, and small amount of bacteria have been survived at 24hr (the death rate:0%)
4. E. coli and Staph. aureus: E. coli continuousiy increased to 3.3x10??/ml at 24hr and Staph. aureus maximaliy increased to 1.3x 10??/ml at 8hr, but rapidly decreased to 1x10/ml at 12hr, and small amount of bacteria are survived at 24hr (the death ratd: 30%)
(The growth of E. coli is superior to staph. aureus).
5. E. coli and Staph. aureus: E. coli rapidly increased to 1.1x10??/ml at 2hr and decrdased to 1.1x10³/ml at 8hr, and slowly increased to 4.0x10??/ml at 24hr, S.marcescens continuously increased to 2.6x 10??/ml at 24hr (the death rate: 50%)
(The growth of E. coli is superior to S. marcescens).
6. Staph. aureus and S. marcescens: Staph. aureus rapidly increased to 2.9x10??/ml at 8hr and decreased to 1.0x10/ml at 8hr, and slowly increased to 4.0x10³/ml, S. marcescens rapidly increased to 1.3x10??/ml at 2hr and slightly decreased to 1.1x 10??/ml at 5hr, and slowly increased to 7.3x10??/ml at 24hr(the death ratd: 50%)
(The growth of S. marcescens is superior to Staph. aureus).
7. E. coli, Staph. aureus, and S. marcescens: E. coli, Staph. aureus, and S.marsescens rapidly increased to 8.8x10??/ml, 6.5x10??/ml, 6.8x10??/ml at 2hr, in respectively, and all strain slightly decreased to 5hr, and showly increased to 2.0x10??/ml, 3.9x10??/ml, 1.7x10??/ml at 24hr, in respectively.(the death rate:60%)
(The growth of E. coli is fastest and followed to S.marcescens, Staph. aureus).
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